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目的建立LC-MS/MS方法测定人血浆中阿罗洛尔的浓度。方法人血浆样品用乙酸乙酯提取后,选用ZORBAX Extend C18(2.1 mm×100 mm,3.5-m)色谱柱,以乙腈-水(80∶20,含0.1%甲酸)为流动相,流速为0.30 m L·min-1,采用电喷雾离子化源,正离子方式,多反应监测(MRM)扫描方式进行监测,用于定量分析的离子反应分别为m/z372.3→315.9(阿罗洛尔)和m/z 267.2→145.0(内标阿替洛尔)。结果血浆中阿罗洛尔的线性范围为0.5~1 000 ng·m L-1(r=0.995 2),定量下限为0.5 ng·m L-1;日内、日间RSD均<15%;低、中、高3个浓度的提取回收率分别为(80.6±1.6)%,(83.2±3.1)%和(87.5±4.5)%。结论该方法快速、灵敏、准确,专属性强,重复性好,适用于人血浆中阿罗洛尔浓度的测定,可应用于阿罗洛尔的血药浓度检测和药动学研究。
Objective To establish a LC-MS / MS method for the determination of arotinolol in human plasma. Methods Human plasma samples were extracted with ethyl acetate. The mobile phase consisted of acetonitrile-water (80:20, containing 0.1% formic acid) and the mobile phase was ZORBAX Extend C18 (2.1 mm × 100 mm, 3.5- m L · min-1. The electrospray ionization source, positive ion mode and multi-reaction monitoring (MRM) were used to monitor the ion reaction for quantitative analysis. The ion reactions were m / z 372.3 → 315.9 ) And m / z 267.2 → 145.0 (internal standard atenolol). Results The linear range of arotinolol was 0.5-1 000 ng · m L-1 (r = 0.995 2), and the lower limit of quantitation was 0.5 ng · m L-1. The intra-day and inter-day RSD were all less than 15% The recoveries were (80.6 ± 1.6)%, (83.2 ± 3.1)% and (87.5 ± 4.5)%, respectively. Conclusion The method is rapid, sensitive, accurate, specific and reproducible. It is suitable for the determination of arotinolol concentration in human plasma and can be applied to the study of arotinolol plasma concentration and pharmacokinetics.