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目的探讨缬沙坦对脓毒症大鼠的早期干预作用。方法大鼠72只,随机分为4组:正常组、模型组、缬沙坦干预组、生理盐水对照组。每组分别于腹腔注射后2h、4h、8h三个时间点(T_2、T_4、T_8)进行相关检测。正常组仅予生理盐水3 mg/kg腹腔注射,模型组予腹腔注射脂多糖(LPS,3 mg/kg),缬沙坦干预组在腹腔注射LPS后10 min予缬沙坦(5 mg/kg)灌胃,生理盐水对照组在腹腔注射LPS后10 min予等量生理盐水灌胃。分别于腹腔注射LPS或生理盐水后相应时间点处死动物,取心脏血,采用ELISA法检测各组血清中肿瘤坏死因子(TNF)-α、血管内皮生长因子(VEGF)、可溶性L-选择素(sL-seleetin)的浓度。结果大鼠腹腔注射LPS后,血清TNF-α、VEGF、sL-selectin的浓度均有明显升高。缬沙坦干预组与模型组各时间点相比,上述增高的细胞因子浓度显著降低(P<0.05)。结论缬沙坦能显著降低脓毒症大鼠增高的血清TNF-α、VEGF、sL-seleetin的浓度水平,通过减少炎症相关介质的释放、抑制白细胞活化、降低毛细血管通透性等机制减轻机体的炎症反应。
Objective To investigate the early intervention of valsartan in septic rats. Methods 72 rats were randomly divided into 4 groups: normal group, model group, valsartan intervention group and saline control group. The rats in each group were detected at 2, 4 and 8 h after injection (T_2, T_4 and T_8) respectively. Rats in the normal group were injected intraperitoneally with saline 3 mg / kg, LPS (3 mg / kg) was injected intraperitoneally into the model group, valsartan (5 mg / kg) ) Gavage, normal saline control group in the intraperitoneal injection of LPS after 10 min to give the same amount of saline gavage. The animals were sacrificed at the corresponding time point after intraperitoneal injection of LPS or saline, and the blood was collected for determination of the levels of tumor necrosis factor (TNF) -alpha, vascular endothelial growth factor (VEGF), soluble L-selectin sL-seleetin). Results After intraperitoneal injection of LPS, the concentrations of serum TNF-α, VEGF and sL-selectin were significantly increased. The valsartan intervention group compared with the model group at each time point, the above elevated cytokine concentration was significantly reduced (P <0.05). Conclusion Valsartan can significantly reduce the serum levels of TNF-α, VEGF and sL-seleetin in septic rats, and reduce the release of inflammatory mediators, the inhibition of leukocyte activation and the decrease of capillary permeability Inflammatory reaction.