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目的:建立舒筋活血片中4-甲氧基水杨醛的HPLC测定方法。方法:Zorbax C_(18)柱(250mm×4.6mm,5μm);流动相为甲醇-水-磷酸(70:30:0.1);流速为1.0 ml·min~(-1);柱温25℃;检测波长为278nm。结果:4-甲氧基水杨醛在0.44～3.52μg范围内与峰面积有良好的线性关系(r=0.9999),平均回收率为104.2%,RSD=1.7%。结论:本法简便、灵敏、准确、重现性好,可用于舒筋活血片的质量控制。
Objective: To establish a HPLC method for the determination of 4-methoxysalicylaldehyde in Shujin Huoxue Tablets. Method: Zorbax C 18 column (250mm×4.6mm, 5μm); mobile phase methanol-water-phosphoric acid (70:30:0.1); flow rate 1.0 ml·min -1; column temperature 25°C; The detection wavelength is 278 nm. Results: There was a good linear relationship between peak area and 4-methoxysalicylaldehyde in the range of 0.44-3.52 μg (r=0.9999). The average recovery was 104.2% and RSD was 1.7%. Conclusion: This method is simple, sensitive, accurate and reproducible. It can be used for the quality control of Shujin Huoxue tablets.