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目的 :研究肝细胞生长因子对大鼠肝细胞原代培养的影响。方法 :利用胶原酶原位二步灌注法分离大鼠肝细胞 ,接种并预培养 1 .5 h后 ,用含不同浓度 HGF的培养基继续培养至 48h,MTT比色法检测肝细胞生长因子对原代培养大鼠肝细胞增殖的影响。结果 :在所设加样量范围内 ,培养基所含 HGF为 1 0 μg/m L时 ,原代培养大鼠肝细胞的增殖出现最高峰。结论 :HGF对原代培养的大鼠肝细胞的增殖有明显的影响 ,但其作用效果无剂量依从关系
Objective: To study the effect of hepatocyte growth factor on primary culture of rat hepatocytes. Methods: Rat hepatocytes were isolated by collagenase in situ two-step perfusion method. After being inoculated and pre-cultured for 1.5 h, cultured with different concentrations of HGF for 48 h, MTT assay was used to detect hepatocyte growth factor Effect of Primary Culture on Rat Hepatocyte Proliferation. Results: When the culture medium contained 10 μg / ml of HGF in the sample volume range, the highest peak appeared in the proliferation of primary cultured rat hepatocytes. CONCLUSION: HGF has a significant effect on the proliferation of primary cultured rat hepatocytes, but its effect has no dose-dependent relationship