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选用培矮６４Ｓ（代表农垦５８Ｓ）、安农Ｓ－１、衡农Ｓ－１、５４６０Ｓ、新光Ｓ等５种光温敏核不育系，于１９９５年在海南春季可育期内互交，夏季在合肥种植，以花粉败育率、套袋结实率、自然结实率为育性指标，进行核不育基因等位性研究，结果表明：培矮６４Ｓ、安农Ｓ－１、衡农Ｓ－１、５４６０Ｓ的核不育基因位点互不等位，新光Ｓ与培矮６４Ｓ基因位点相同。另选用农垦５８Ｓ转育成的一批籼粳不育系，以培矮６４Ｓ为桥梁亲本，粳型不育系直接与农垦５８Ｓ配组，籼型不育系与培矮６４Ｓ配组，研究方法同上，结果表明参试的不育系均与农垦５８Ｓ等位
Five light-thermo-sensitive genic male sterile lines Pei'ai 64S (on behalf of Nongken 58S), Annong S-1, Hengnong S-1, 5460S and Shin Kong S were selected and crossed each other in the fertile season of Hainan in 1995, The results showed that Pei'ai 64S, Annong S-1 and Hengnong S were the most susceptible to fertility. The results showed that: Pei'ai 64S, Annong S-1, Hengnong S The -1,5460S genomic loci are not equal to each other, and Shin Kong S is identical to Pei'ai 64S gene loci. In addition, a group of indica-japonica CMS lines transformed by Nongken 58S were selected. Pei'ai 64S was used as the bridge parent. The japonica CMS line was directly matched with Nongken 58S, indica sterile line and Pei'ai 64S, The results showed that all tested sterile lines were all the same with Nongken 58S