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为了从化学成分上研究长柱重楼是否具有重楼药材代用品的开发价值,该研究利用UPLC-Q-TOF MS比较了长柱重楼和滇重楼的化学成分,并进一步利用HPLC-UV同时测定了77株不同来源的长柱重楼中8种重楼皂苷的含量。结果显示长柱重楼和滇重楼的化学成分相似,长柱重楼化学成分更为复杂,鉴定的22个主要共有峰均为甾体类化合物。不同株长柱重楼中重楼皂苷的含量具有一定的差异,其中药典规定4种甾体皂苷(重楼皂苷Ⅰ,Ⅱ,Ⅵ,Ⅶ)总量最高的为3.30%,最低的为0.068%,8种甾体皂苷总量最高的为6.18%,最低的为0.71%。长柱重楼中78%的样品符合《中国药典》要求,整体质量较为稳定,可以作为重楼替代品种进行研究开发。
In order to study the chemical constituents of the long column re-build whether there is heavy floor herbs alternative value of development, the study uses UPLC-Q-TOF MS was compared with the chemical composition of the reticulate and the reticulopapillant, and further use of HPLC-UV At the same time, the content of eight kinds of polysaccharide in tall reed of different sources were determined. The results show that the chemical composition of the reticulated column and the reticulated column are similar. The chemical composition of the long column reticulated column is more complicated. The 22 main common peaks identified are all steroidal compounds. There were some differences in the contents of gypsum saponins in different length of column. Among them, the total amount of four steroidal saponins (polysaccharide Ⅰ, Ⅱ, Ⅵ, Ⅶ) in the pharmacopoeia was 3.30% and the lowest was 0.068% , The highest total amount of eight kinds of steroidal saponins was 6.18%, the lowest was 0.71%. 78% of the samples in the long column re-building complied with the requirements of the “Chinese Pharmacopoeia”, the overall quality is relatively stable, and it can be used as a substitute for heavy building for research and development.