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目的探讨基质细胞衍生因子-1α(SDF-1α)、骨桥蛋白(OPN)、变异的CD44受体(CD44v6)和抗酒石酸酸性磷酸酶5b(TRACP-5b)在多发性骨髓瘤(MM)发病中的作用。方法用ELISA法检测了32例MM患者和15例对照骨髓单个核细胞(MNCs)和骨髓基质细胞(BMSCs)的SDF-1α、OPN、CD44v6和TRACP-5b水平。结果 MNCs产生的SDF-1α、OPN、CD44v6水平在初发和复发组、病情稳定MM组和对照组显著下降(P<0.05)。9例初发和复发患者的BMSCs与人骨髓瘤细胞系U266加入rhIL-6混合培养后,SDF-1α水平明显增高(P<0.05)。SDF-1α、OPN、TRACP-5b水平在MM患者骨质破坏≥3处、<3处和对照组显著下降(P<0.05)。结论 SDF-1α、OPN和CD44v6水平升高与MM发病或病情进展、骨质破坏有关。
Objective To investigate the role of SDF-1α, OPN, CD44v6 and TRACP-5b in the pathogenesis of multiple myeloma (MM) In the role. Methods The levels of SDF-1α, OPN, CD44v6 and TRACP-5b in 32 MM patients and 15 control bone marrow mononuclear cells (MNCs) and bone marrow stromal cells (BMSCs) were detected by ELISA. Results The levels of SDF-1α, OPN and CD44v6 produced by MNCs were significantly decreased in the initial and recurrent group, stable MM group and control group (P <0.05). SDF-1α was significantly higher in BMSCs of 9 patients with initial relapse and in U266 cell line mixed with rhIL-6 (P <0.05). The levels of SDF-1α, OPN and TRACP-5b were significantly lower than those in the control group (P <0.05). Conclusion The elevated levels of SDF-1α, OPN and CD44v6 are associated with the pathogenesis of MM or the progression of bone destruction.