以栽培甜菜的未授精胚珠或胚为外植体，在ＭＳ附加ＮＡＡ和ＢＡ的培养基上诱导愈伤组织，选择胚性愈伤组织进行继代培养，从该愈伤组织分离原生质体并以液体浅层法或半固体琼脂糖法培养在一系列培养基上，植板率为０．０１％～１．２％．再生愈伤组织形成后，转入固体培养基（ＭＳＢ）上培养，再转入分化培养基上分化出苗，分化率可达２．５％，由愈伤组织上切下分化苗在生根培养基上很容易诱导生根。 The non-inseminated ovules or embryos were used as explants to induce callus on medium supplemented with MSA and NAA. Embryogenic callus was selected for subculture and protoplasts were isolated from the callus Liquid shallow method or semi-solid agar culture in a series of medium, the plating rate of 0.01% to 1.2%. After the regenerated callus was formed, it was transferred to solid medium (MSB) for culture, and then differentiated into differentiation medium on the differentiation medium. The rate of differentiation was up to 2.5%. The callus from the callus was seeded in rooting medium It is easy to induce rooting.