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B_(301)是一株分离自空气中的假单胞杆菌(pseudomonas sp.)。室内测定表明,它能抑制稻瘟病菌的分生孢子萌发和菌丝生长。病菌分生孢子在B_(301)发酵液的2倍稀释液中完全不能萌发,40倍稀释液中的萌发率为35.58％,比对照下降59.4％。B_(301)发酵液稀释140倍时,对病菌菌丝生长的抑制率为52.1％,其抑制菌丝生长的最大稀释度为4480倍。B_(301)发酵液中的抗菌物质具有较强的耐热性,在80℃下,60min仍保持原有活性,但当温度上升到100℃后则不稳定,10min内活性显著下降,80min后完全失活。田间防治试验表明,B_(301)发酵液防治稻瘟病的效果为45％左右,与对照药剂20％三环唑可湿性粉剂500倍液防效相当。此外,B_(301)还能有效抑制棉花炭疽病菌和玉米小斑病菌的生长。
B 301 is isolated from the air in the Pseudomonas sp. Laboratory tests show that it can inhibit the conidia germination and mycelial growth of Magnaporthe grisea. The pathogen conidia could not germinate completely in the two times dilution of B_ (301) fermentation broth, the germination rate was 35.58% in 40 times dilution and 59.4% lower than the control. When the broth of B_ (301) was diluted by 140 times, the inhibition rate of mycelium growth was 52.1% and the maximum dilution of mycelial growth was 4480 times. Antibacterial substances in B_ (301) fermentation broth had strong heat resistance, and maintained their original activity at 60 ° C for 60 minutes, but remained unstable after the temperature rose to 100 ° C. The activity decreased significantly after 10 minutes. After 80 minutes Completely inactivated. Field control experiments showed that the effect of blasting broth of B 301 was about 45%, which was equivalent to that of 500% of 20% tricyclazole WP. In addition, B_ (301) can also effectively inhibit the growth of C. anthracis and C. turcica.