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目的:研究幽门螺杆菌(HP)感染与胆石形成的关系。方法:采用聚合酶链免疫吸附法(ELISA)对胆结石患者的血清及胆汁免疫球蛋白IgG、IgA、IgM标本进行检测,对血清学及胆汁IgG均阳性患者的胆汁,采用免疫印迹法(Western blot)进行HP感染相关蛋白检测。结果:胆结石组50例患者中血清学及胆汁IgG均阳性者29例(58.0％)。另取非胆结石组13例作为对照。胆结石组与非胆结石组血清及胆汁HP免疫印迹检测,均可检出4种主要HP感染相关蛋白,但胆结石组血清及胆汁中Ure A检出率较非胆结石组高。结论:胆汁中存在多种HP感染相关蛋白,并可能参与胆结石的形成。
Objective: To study the relationship between Helicobacter pylori (HP) infection and gallstone formation. Methods: Serum and bile immunoglobulin IgG, IgA and IgM specimens of gallstone patients were detected by polymerase chain reaction (ELISA). Serum and bile of patients with positive bile IgG were detected by Western blotting blot for HP infection-related protein detection. Results: Among the 50 patients with gallstones, 29 cases (58.0%) had positive serology and bile IgG. Another non-gallstone group of 13 cases as a control. In the gallstone group and the non-gallstone group, serum and bile HP immunoblotting detected four major HP infection-related proteins, but the detection rate of Ure A in serum and bile of gallstone group was higher than that in non-gallstone group. Conclusion: There are many HP infection-related proteins in bile and may be involved in the formation of gallstones.