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目的研究表没食子儿茶素没食子酸酯(EGCG)联合姜黄素对人卵巢癌细胞株A2780增殖的抑制作用及其可能机制。方法将A2780细胞随机分为空白对照组(A组)、EGCG 10μmol/L处理组(B组)、姜黄素5μmol/L处理组(C组)和EGCG 10μmol/L+姜黄素5μmol/L联合处理组(D组)。采用MTT法检测A2780细胞存活率,5-乙炔基-2′-脱氧尿苷(EdU)荧光染色检测细胞DNA复制活性,平板集落形成实验观察单个细胞的生存能力,Western blot法检测细胞周期蛋白B1(Cyclin B1)和Cyclin D1的表达。结果 EGCG和姜黄素均呈剂量依赖性地抑制A2780细胞增殖(P<0.05)。与A、B、C组相比,D组细胞存活率、EdU染色阳性细胞数、细胞集落形成率、Cyclin B1和Cyclin D1表达均降低(P<0.05或P<0.01)。结论 EGCG联合姜黄素能明显抑制A2780细胞增殖;其机制可能与诱导细胞周期阻滞有关。
Objective To study the inhibitory effect of epigallocatechin-3-gallate (EGCG) and curcumin on the proliferation of human ovarian cancer cell line A2780 and its possible mechanism. Methods A2780 cells were randomly divided into blank control group (A group), EGCG 10μmol / L group (B group), curcumin 5μmol / L group (C group) and EGCG 10μmol / L + curcumin 5μmol / (Group D). The viability of A2780 cells was detected by MTT assay and the DNA replication activity was detected by EdU staining. The viability of individual cells was observed by plate colony formation assay. The expression of cyclin B1 (Cyclin B1) and Cyclin D1 expression. Results EGCG and curcumin all inhibited the proliferation of A2780 cells in a dose-dependent manner (P <0.05). Compared with groups A, B and C, the cell survival rate, the number of EdU positive cells, the colony formation rate and the expressions of Cyclin B1 and Cyclin D1 in group D were all decreased (P <0.05 or P <0.01). Conclusion EGCG combined with curcumin can significantly inhibit A2780 cell proliferation; its mechanism may be related to the induction of cell cycle arrest.