作者等(1988)用巴西利什曼原虫复合种制备了B_1和B_2两个寡核苷酸引物,其对该复合种内的种有高度特异性,并以其用于PCR检测。本文报告用PCR对哥伦比亚疑似利什曼病的病人检测的同时,还与其当前临床上用于检查活组织的方法,包括直接涂片、穿刺物培养、活组织培养及活组织金色仓鼠鼻部接种等作了比较。20例可疑病人年龄1—52岁,除1例有粘膜症状外,余均为皮肤 The authors (1988) prepared two oligonucleotide primers, B_1 and B_2, from Leishmania braziliensis, which are highly specific to the species in the complex and used for PCR detection. This article reports the use of PCR for the detection of patients with suspected leishmaniasis in Colombia as well as their current clinical practice for examining biopsies, including direct smear, puncture culture, biopsy, and nasal inoculation of living golden hamsters For comparison. 20 cases of suspicious patients aged 1-52 years, except for 1 case of mucosal symptoms, the remaining are the skin