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目的建立胚胎后肾大网膜内移植大鼠模型,并探讨其在受者体内生长、发育情况。方法取孕16d(E16)和17d(E17)的SD大鼠胚胎,切取胚胎后肾,以组氨酸色氨酸酮戊二酸盐液(HTK液)保存3d,然后移植到切除单侧肾脏的成年SD大鼠的大网膜内,另设未经保存的E16胚胎后肾直接移植对照组。术后给予环孢素A皮下注射,术后3～4周后开腹观察器官形成情况;术后8周,切除受者自体肾脏,观察移植后肾的组织学形态,测定后肾功能。结果移植后3周,移植后肾肾单位、集合管及输尿管的结构正常,组织中少有淋巴细胞浸润,电镜显示移植后肾发育的肾血管球细胞及基底膜、近端肾小管、远端肾小管和集合管上皮细胞超微结构正常。移植后8周,移植后肾的湿重、体积、分泌尿量及内生肌酐清除率与对照组比较,差异无统计学意义(P>0.05)。结论E16、E17胚胎后肾大网膜内移植,并辅以环孢素A皮下注射,可以形成器官,并发挥功能。
OBJECTIVE: To establish a rat model of intraorbital nevus omentum transplantation and to investigate its growth and development in recipients. Methods The SD rat embryos at 16 days (E16) and 17 days (E17) were obtained and the embryonic kidney was excised. The cells were stored for 3 days with histidine tryptophan ketoglutarate (HTK) solution and then transplanted into the unilateral kidney Of the adult SD rats omentum, another set of non-preserved E16 embryo kidney transplantation group. Postoperatively, cyclosporin A was given subcutaneously. After 3 to 4 weeks, the organs were laparotomized for observation of organ formation. After 8 weeks, the recipients were sacrificed and their renal morphology was observed. The renal function was observed after transplantation. Results Three weeks after transplantation, the structure of renal allograft, collecting duct and ureter after transplantation was normal with few infiltration of lymphocytes in the tissues. Electron microscopy showed that renal allograft nephrocytes and basement membrane, proximal tubule, distal end Tubular and collecting duct epithelial cells normal ultrastructure. At 8 weeks after transplantation, there was no significant difference in wet weight, volume, urine output and endogenous creatinine clearance after transplantation between the two groups (P> 0.05). Conclusion The E16 and E17 embryos are implanted in the omentum of the posterior kidney and subcutaneously injected with cyclosporin to form organs and function.