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目的：探讨少量多次异基因骨髓移植的ＧＶＨＤ反应。方法：采用不同少量异基因骨髓细胞的多次尾静脉注射。观察大小便，皮毛，体位及死亡率等ＧＶＨＤ反应一般表现。移植后不同天数进行体内混合淋巴细胞反应及嵌合体测定。外周血象及骨髓有核细胞按常规方法计数。半固体琼脂法进行骨髓ＣＦＵ－ＧＭ测定。肝、脾、小肠及皮肤病理学检查判断ＧＶＨＤ反应程度。外周血Ｌ６１５细胞及脾脏Ｌ６１５细胞比例判断ＧＶＬ作用。结果：一次性大剂量组ＧＭＨＤ反应表现明显，单纯多次异基因骨髓细胞移植的ＧＶＨＤ反应不明显，而少量多次骨髓移植组则ＧＶＨＤ反应强度界于两者之间，并且具有较好的造血恢复作用。在ＧＶＬ作用方面，一次性大剂量骨髓移植组产生较强的近期抗白血病作用，但多死于ＧＶＨＤ反应。少量多次骨髓移植１×１０６骨髓细胞及 １×１０６脾单个核细胞组抗白血病作用较强，３０ ｄ生存率达 ６０％，死亡原因主要为白血病。结论：少量多次骨髓移植可以减轻ＧＶＨＤ反应，保留部分ＧＶＬ作用。
Objective: To investigate the GVHD response in a small number of allogeneic bone marrow transplantation. Methods: A small amount of allogeneic myeloid cells were injected multiple times through the tail vein. Observe the general performance of GVHD response such as urine, fur, body position and mortality. Different days after transplantation, mixed lymphocyte reaction in vivo and chimerism assay. Peripheral blood and bone marrow nucleated cells by conventional methods count. Semi-solid agar method for bone marrow CFU-GM determination. Liver, spleen, small intestine and skin pathological examination to determine the extent of GVHD reaction. Peripheral blood L615 cells and spleen L615 cell ratio to determine the role of GVL. Results: The GMHD response in the one-time high-dose group was obvious. The response to GVHD was not obvious only in multiple allogeneic bone marrow transplantation. However, the GVHD response intensity in a small number of multiple bone marrow transplantation group was between the two and had good hematopoietic Restore effect. In the role of GVL, one-time high-dose bone marrow transplantation group has a strong recent anti-leukemia effect, but more death from GVHD response. A small number of multiple bone marrow transplantation 1 × 106 bone marrow cells and 1 × 106 spleen mononuclear cell group stronger anti-leukemia, 30-day survival rate of 60%, the main cause of death was leukemia. Conclusion: A small number of multiple bone marrow transplantation can reduce GVHD response, retain some of the role of GVL.