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系统地研究了在碱性介质和阳离子表面活性剂CTMAB存在下,NaClO氧化异硫氰酸荧光素 牛血红蛋白标记物的化学发光行为和化学发光反应的条件,提出了反相流动注射化学发光测定牛血红蛋白的新方法。该方法测定牛血红蛋白的线性范围为0.26～12.8μg/mL,检出限为0.128μg/mL。
The chemiluminescence and chemiluminescence reaction conditions of the oxidation of fluorescein isothiocyanate isothiocyanate (BIS) in the presence of alkaline medium and cationic surfactant CTMAB were systematically investigated. The reversed-phase flow-injection chemiluminescence New method of hemoglobin. The linear range of this method for the determination of bovine hemoglobin was 0.26 ~ 12.8μg / mL with the detection limit of 0.128μg / mL.