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目的研究马来酸桂哌齐特(cinepazide maleate,CM)对大鼠急性脑缺血后IL-1β、IL-6的表达及神经功能的影响。方法成年雄性SD大鼠48只,采用随机数字表法分为正常组、假手术组、缺血再灌注组及CM治疗组,在缺血再灌注后第2、7天对脑组织进行HE染色、测定脑组织含水量及采用免疫组化法测定皮质IL-1β、IL-6的表达,并对动物进行神经功能评分。结果脑缺血再灌注后第2天,CM治疗组与缺血再灌注组比较,脑内有较多的神经细胞存活,IL-1β、IL-6的表达被明显抑制(P<0.01),但是CM治疗组脑组织含水量及神经功能评分与缺血再灌注组比较无明显改善(P>0.05)。至缺血再灌注后第7天,缺血再灌注组IL-1β、IL-6的表达较早期有所降低(P<0.01),同时伴随神经功能的改善(P<0.05),CM治疗组的改善更为明显(P<0.01)。结论 CM对大鼠局灶性脑缺血再灌注损伤有一定的脑保护作用,其作用机制可能与CM降低脑内IL-1β、IL-6的表达有关。
Objective To investigate the effects of cinepazide maleate (CM) on the expression of IL-1β, IL-6 and neurological function after acute cerebral ischemia in rats. Methods Forty-eight adult male Sprague Dawley rats were randomly divided into normal group, sham operation group, ischemia-reperfusion group and CM treatment group. HE staining was performed on the 2nd and 7th day after ischemia-reperfusion The water content of brain tissue was measured. The expression of IL-1β and IL-6 in cortex was detected by immunohistochemical method. The neurological function was evaluated. Results On the second day after cerebral ischemia-reperfusion, compared with the ischemia-reperfusion group, the number of neurons survived in the CM group and the levels of IL-1β and IL-6 were significantly inhibited (P <0.01) However, the score of brain water content and neurological function in CM treatment group had no significant improvement compared with ischemia-reperfusion group (P> 0.05). The expression of IL-1β and IL-6 in ischemia-reperfusion group was lower than that in the early stage (P <0.01), and the neurological function was improved at the 7th day after ischemia / reperfusion (P <0.05) The improvement is more obvious (P <0.01). Conclusion CM may have protective effect on focal cerebral ischemia-reperfusion injury in rats. Its mechanism may be related to the decrease of brain IL-1β and IL-6 expression by CM.