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1 材料与方法 用本院饲养的C_(57)、5～6W龄的雌性小鼠50只,随机分为对照和治疗两组。引流熊胆粉经乙醇冷浸热提后制成注射剂。于中毒前6d开始给药,剂量为0.4mg/0.2ml/次,1次/d,ip。对照组以同样方法给予等量生理盐水。连续6d后,两组动物一次腹腔注射50％CCl_40.3ml/只予以中毒。中毒后两组动物继续分别给予熊胆注射液和生理盐水,连续6d。在中毒前及中毒后1、2、4和第7d分批处死,应用常规方法观察中毒小鼠血清谷丙转氨酶(GPT)和总胆红素(T—BIL)以及外周血T淋巴细胞亚群的变化。
1 Materials and Methods Fifty C_(57) and 5-6W female mice were randomly assigned to control and treatment groups. Drainage bear bile powder was cold ethanol soaked and made into injections. Dosing was started 6 days before poisoning at a dose of 0.4 mg/0.2 ml/time, once/d, ip. The control group was given the same amount of physiological saline in the same manner. After 6 days in a row, the animals in the two groups were given an intraperitoneal injection of 50% CCl_40.3 ml/only. After poisoning, the animals in the two groups continued to receive bear bile injection and saline, respectively, for 6 days. Before sacrifice and 1, 2, 4 and 7d after poisoning, they were sacrificed in groups and routinely used to observe serum GPT, total bilirubin (T-BIL) and peripheral blood T lymphocyte subsets in poisoned mice. The change.