论文部分内容阅读
目的探讨Livin靶向RNA干扰对SGC7901细胞株生物学特性的影响,探索胃癌基因治疗的新途径。方法设计和构建Livin特异性的siRNA真核表达载体,转染SGC7901细胞,应用RT-PCR分析LivinmRNA的表达情况,采用MTT法检测细胞的生长状况,通过流式细胞仪进行细胞凋亡的检测。结果构建真核表达载体p-siRNA1并转染SGC7901细胞后,RT-PCR结果显示2条Livin siRNA真核表达质粒均能有效抑制Livin mRNA的转录表达,干扰组细胞与未转染组细胞比较,生长受到明显抑制,凋亡率明显增加。结论成功构建了Livin干扰真核表达载体,siRNA重组体能有效抑制人胃癌细胞Livin mRNA表达,并抑制胃癌细胞生长,促进其凋亡。
Objective To investigate the effect of Livin targeted RNA interference on the biological characteristics of SGC7901 cell line and explore new ways of gene therapy for gastric cancer. Methods Livin-specific siRNA eukaryotic expression vector was designed and constructed and transfected into SGC7901 cells. The expression of Livin mRNA was analyzed by RT-PCR. The cell growth was detected by MTT assay and the apoptosis was detected by flow cytometry. Results After constructed eukaryotic expression vector p-siRNA1 and transfected into SGC7901 cells, RT-PCR results showed that the two Livin siRNA eukaryotic expression plasmids could effectively inhibit the expression of Livin mRNA. Compared with untransfected cells, Growth was significantly inhibited, the apoptosis rate increased significantly. Conclusion The Livin expression vector was successfully constructed. SiRNA recombinant could effectively inhibit the expression of Livin mRNA and inhibit the growth of gastric cancer cells and promote its apoptosis.