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应用抗建兰花叶病毒(CymMV)的单克隆抗体,建立了快速检测蝴蝶兰病样的免疫斑点法(Dot-ELISA)和组织印迹法(Tissue blot-ELISA)。CymMV单抗稀释8000倍时,Dot-ELISA可检出病毒粗汁液的最大稀释度为1:10240;Tissue blot-ELISA中样品1次平切后1~5次印迹与Dot-ELISA样品1:80稀释结果相当,6~8次印迹与Dot-ELISA1:320稀释结果相当,前8次印迹均可以得到满意的检测效果。Tissue blot-ELISA的灵敏度略低于Dot-ELISA,使用单抗的结果比多抗特异性强,操作更为简便,适合大量兰花样品的快速检测。
The monoclonal antibodies against CymcV were used to establish the Dot-ELISA and Tissue blot-ELISA for the rapid detection of Phalaenopsis. Dot-ELISA detected a maximum dilution of virus crude juice of 1: 10240 when CymMV mAb was diluted 8000-fold; samples of Tissue blot-ELISA were 1 to 5 impressions and Dot-ELISA samples 1: 80 The results of the dilution were equivalent. The results of 6 to 8 blots were equivalent to that of Dot-ELISA 1: 320. The first 8 blots were satisfactory. The sensitivity of Tissue blot-ELISA was slightly lower than that of Dot-ELISA. The results of using the mAb were stronger than that of the polyclonal antibody and the operation was more convenient. It was suitable for the rapid detection of a large number of orchid samples.