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目的通过对PTEN和血管内皮生长因子(vascular endothelial growth factor,VEGF)在贲门癌中表达的研究,结合临床病理特征,探讨PTEN和VEGF的临床意义及相关性。方法应用免疫组织化学S-P法检测100例贲门癌组织及20例正常胃黏膜中PTEN和VEGF的表达。结果贲门癌组织中PTEN蛋白表达率为34.00%(34/100),显著低于正常胃黏膜的表达95%(19/20)(P<0.01),与组织分化程度呈正相关(P<0.05),与淋巴结转移呈负相关(P<0.01)。VEGF在贲门癌中的表达率为75.00%(75/100),显著高于正常胃黏膜的表达10.0%(2/20)(P<0.01),与癌组织浸润深度呈正相关(P<0.05),与淋巴结转移也呈正相关(P<0.05),与组织分化程度呈负相关(P<0.05)。PTEN在贲门癌中的表达与VEGF呈负相关(P<0.01)。结论联合检测PTEN、VEGF有助于提高贲门癌侵袭转移能力的评估,对贲门癌的预后判断具有重要的临床意义。
Objective To investigate the clinical significance and correlation of PTEN and VEGF by investigating the expression of PTEN and vascular endothelial growth factor (VEGF) in cardia carcinoma and clinicopathological features. Methods The expression of PTEN and VEGF in 100 cases of gastric cardia carcinoma and 20 cases of normal gastric mucosa were detected by immunohistochemical S-P method. Results The positive rate of PTEN protein in gastric cardia was 34.00% (34/100), significantly lower than that in normal gastric mucosa (95%, 19/20) (P <0.01), and positively correlated with the degree of differentiation (P <0.05) , But negatively correlated with lymph node metastasis (P <0.01). The positive rate of VEGF in gastric cardia carcinoma was 75.00% (75/100), significantly higher than that in normal gastric mucosa (10.0%) (P <0.01), and positively correlated with the depth of invasion (P <0.05) , Positive correlation with lymph node metastasis (P <0.05) and negative correlation with histological differentiation (P <0.05). The expression of PTEN in cardia cancer was negatively correlated with VEGF (P <0.01). Conclusions The combined detection of PTEN and VEGF is helpful to improve the invasion and metastasis of gastric cardia carcinoma and has important clinical significance for the prognosis of cardia carcinoma.