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探讨重组人促红细胞生成素(recombinant human erythmpoietin,rhEPO)作为血管生长样因子对高氧致新生大鼠支气管肺发育不良(bronchopulmonary dysplasia,BPD)的血管保护作用。将96只新生Wistar大鼠生后1h随机分为4组:(1)空气对照,(2)空气+rhEPO,(3)高氧对照,(4)高氧+rhEPO。第3、第4组置于玻璃氧箱中,持续输入氧气,FIO2=850ml/L,第2、第4组于生后即刻、高氧暴露前30min和生后2d给rhEPO 2400IU/kg背部皮下注射,第1、第3组给予等量生理盐水注射。于生后第3、7及10d采集肺组织标本,光镜下观察肺组织学改变,应用免疫组化测定肺组织织血管内皮标志CD31及肺血管内皮细胞生长因子(vascular endothelial growth factor VEGF)表达的变化。研究发现:与高氧组相比,高氧+rhEPO组大鼠肺组织CD31阳性面积比和VEGF的表达明显增高;3d增高,10d达高峰。结果提示:rhEPO(2400IU/kg)可以促进肺血管的发育和修复,对高氧致新生鼠BPD有血管保护作用。
To investigate the protective effect of recombinant human erythropoietin (rhEPO) as a vascular growth factor on bronchopulmonary dysplasia (BPD) induced by hyperoxia in neonatal rats. Ninety-six neonatal Wistar rats were randomly divided into four groups at 1 hour after birth: (1) air control, (2) air + rhEPO, (3) hyperoxia control, (4) hyperoxia + rhEPO. Groups 3 and 4 were placed in glassy oxygen chamber and oxygen was continuously infused. FIO2 = 850ml / L, groups 2 and 4 were given rhEPO 2400IU / kg immediately after birth, Injections, groups 1 and 3 were given the same amount of saline injection. Lung tissue samples were collected at 3, 7 and 10 days after birth and the lung histological changes were observed under light microscope. The expression of CD31 and VEGF in lung tissue were detected by immunohistochemistry The change. The results showed that compared with hyperoxia group, the expression of CD31 positive area and VEGF in lung tissue of rats in hyperoxia + rhEPO group were significantly higher than those in hyperoxia group, and increased in 3d and reached the peak in 10 days. The results suggest that: rhEPO (2400IU / kg) can promote pulmonary vascular development and repair, hypoxic-induced neonatal rat BPD has a vascular protective effect.