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幽门螺杆菌(HP)不仅存在于胃粘膜细胞表面,而且也存在于胃液中。本文用合成的DNA(sDNA)片段,进行竞争性多聚酶链反应(cPCR),定量检测胃液中HP,并与组织学检查、细菌培养、快速尿素酶试验(PUT)、~(13)C尿素呼吸试验(~(13)C-UBT)和胃液中尿素/氨(U/A)比测定的结果比较。受检的190例有胃溃疡106例、十二指肠溃疡79例、慢性胃炎5例,皆经内镜证实,其中46例还做了~(13)-UBT。患者先采静脉血测定血清尿素量以除外氮血症,随即行胃十二指肠镜检,并抽取胃液10ml,然后从胃窦及胃体部取数个活组织检查标本,供RUT、细菌培养及组织学检查。从100μl胃液抽提细菌DNA,按Clayton等设计的引物对HPU18和HPU54扩增的产物为132bp,因其退火温
Helicobacter pylori (HP) is present not only on the surface of gastric mucosal cells, but also in gastric juice. In this paper, competitive polymerase chain reaction (cPCR) was performed with synthetic DNA (sDNA) fragments and quantitatively detected HP in gastric juice. The results of histological examination, bacterial culture, rapid urease test (PUT), ~ (13) C urea respiration The results of the test (~ (13) C-UBT) and the urea / ammonia (U / A) ratio in gastric juice were compared. Of the 190 cases examined, there were 106 cases of gastric ulcer, 79 cases of duodenal ulcer and 5 cases of chronic gastritis. All of them were confirmed by endoscopy, of which 46 cases had ~ (13) -UBT. Patients first measured venous blood serum urea to exclude azotemia, followed by gastroduodenoscopy, and gastric juice 10ml, and then take a few from the gastric antrum and gastric biopsy specimens for RUT, bacteria Culture and histological examination. Bacterial DNA was extracted from 100 μl of gastric juice. The amplified product of HPU18 and HPU54 according to the primers designed by Clayton et al. Was 132 bp, because of its annealing temperature