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AIM:To investigate the hemostasia effect of hemostasis sponge of collagen and chitosan compound invitro and in vivo.METHODS:The hemostasis sponge of collagen and chitosan compound was made by mixing collegen I extracted by enzymelytic methods and crosslinked by glutaric dialdeiyde and then lyophilized.The New zealand rabbit were created with a 1 cm× 1 cm wound in the center artery of the ear.The center artery of the ear were transected and record the hemostasis time since pressured with hemostasis sponge of collagen and chitosan compound and observe its effects.In other experiements,1 cm× 1 cm bleeding wound were established in the liver of the rabbit.Then pressured with hemostasis sponge of collagen and chitosan compound on the wounds and record the hemostasis time; The four corner of the hemostasis sponge of collagen and chitosan compound were sutured to investigate the absorption situation with the control of gelatin treated with same procedures.RESULTS:The bleeding time using hemostasis sponge of collagen and chitosan compound was 120 seconds compared with 600 seconds of using gelatin,and the hemostasis time using hemostasis sponge of collagen and chitosan compound was 40 seconds compared with 75 seconds of using gelatin.Both the hemostasis sponge of collagen and chitosan compound and gelatin can be degradated within 5 months.CONCLUSION:Hemostasis effect of hemostasis sponge of collagen and chitosan compound is better than that of gelatin and it has an promising future in surgery.