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目的 :探讨人肝硬化肝组织是否存在卵圆细胞。方法 :对 30例人肝细胞肝癌手术标本的癌旁肝硬化组织行常规组织学观察 ,并用胆管上皮分化标记CK7和肝细胞分化标记白蛋白对以上组织作免疫组化染色 ,同时对其中 10例作了超微结构观察 ,5例作免疫电镜标记和观察。另外对 5例正常肝脏和 5例肝炎后肝硬化组织同时作了光镜、免疫组化和电镜观察 ,对 2例肝炎后肝硬化作了免疫电镜标记和观察。结果 :癌旁肝硬化组织的 30例光镜和 10例电镜观察以及 5例肝炎肝硬化光、电镜观察 ,均可在再生的肝细胞结节边缘见到散在的小细胞和增生的小胆管样结构。这些小细胞和增生的小胆管内少数小细胞 ,为卵圆形 ,体积较小 ,核大 ,胞质少 ,胞质内含较多的游离核糖体 ,仅含少量粗面内质网和线粒体 ,胞质内可见张力微丝结构 ,这类细胞与邻近细胞间均有细胞间连接。免疫电镜示 ,卵圆细胞均表达CK7和白蛋白 ,但有些细胞内表达CK7多些 ,有些细胞则表达白蛋白多些。在正常肝组织内未见到类似细胞。结论 :与动物实验性肝癌模型肝组织一样 ,人类肝硬化肝组织中也存在同样细胞形态和免疫表型特点的卵圆细胞。结果支持卵圆细胞可能为肝前体细胞或干细胞的假设
Objective: To investigate the existence of oval cells in human liver cirrhosis. Methods: The histopathological changes of pericarcinomatous cirrhosis in 30 cases of hepatocellular carcinoma (HCC) were observed by histology. The above tissue was stained immunohistochemically with cholangiocarcinoma differentiation marker CK7 and hepatocyte differentiation marker, and 10 of them The ultrastructure was observed, and 5 cases were marked by immunoelectron microscopy. In addition, 5 cases of normal liver and 5 cases of posthepatitic cirrhosis were made by light microscopy, immunohistochemistry and electron microscopy. Immuno-electron microscopy was performed on 2 cases of post-hepatitis cirrhosis. Results: 30 cases of adjacent liver cirrhosis by light microscopy and 10 cases of electron microscopy and 5 cases of cirrhosis of liver cirrhosis light and electron microscopy, can be seen in the regenerated liver cell nodules scattered scattered small cells and proliferative small bile duct like structure. These small cells and proliferative small bile duct small number of small cells, oval, smaller, large nucleus, less cytoplasm, cytoplasm contains more free ribosomes, only a small amount of rough endoplasmic reticulum and mitochondria , The cytoplasm can be seen in the structure of tension microfilaments, such cells and adjacent cells have intercellular connection. Immunoelectron microscopy showed that oval cells expressed CK7 and albumin, but some cells expressed more CK7, while others expressed more albumin. No similar cells were found in normal liver tissue. Conclusions: Like the experimental liver cancer model in animals, there are also oval cells with the same cell morphology and immunophenotype in human liver cirrhosis. The results support the hypothesis that oval cells may be liver precursor cells or stem cells