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目的 通过对胃癌组织中凋亡细胞的原位观察和Bcl 2、Bcl XL、Bcl XS/L、BAX和Mcl 1的表达状态的研究 ,探讨不同病理类型胃癌组织细胞凋亡情况及Bcl 2家族凋亡调控基因蛋白在胃癌组织中的表达对细胞凋亡的影响。方法 5 8例胃癌手术根治标本 ,分别进行凋亡细胞的原位检测 (TUNEL)和Bcl 2、Bcl XL、Bcl XS/L、BAX和Mcl 1蛋白的免疫组化染色。结果 高、中分化腺癌的细胞凋亡指数明显高于低分化和未分化腺癌 (P <0 .0 5 ) :Bcl 2、Bcl XL、Mcl 1抗原表达阳性的胃癌组织细胞凋亡指数明显低于三种抗原表达阴性的胃癌组织 (P <0 .0 1) ,而BAX、Bcl XS/L抗原表达阳性的胃癌组织细胞凋亡指数明显高于二种抗原表达阴性的胃癌组织 (P <0 .0 1) ;Bcl 2、Bcl XL、BAX和Bcl XS/L在高、中分化腺癌中的表达明显高于低分化腺癌和未分化腺癌 (P <0 .0 1) ,而Mcl 1在低分化腺癌中的表达明显高于高、中分化腺癌(P <0 .0 5 )。结论 高、中分化腺癌的细胞凋亡率高 ,促凋亡基因表达阳性的胃癌组织细胞凋亡率高。Bcl 2、Bcl XL、BAX和Bcl XS/L可能与肠型胃癌的发生有关 ,而Mcl 1主要作用于弥漫型胃癌。
Objective To investigate the apoptotic cells in gastric cancer tissues in situ and the expression of Bcl-2, Bcl XL, Bcl XS/L, BAX and Mcl 1 in order to investigate the apoptosis of gastric cancer tissues and Bcl 2 family withering in different pathological types. Expression of Death Regulating Gene Protein in Gastric Carcinoma and Its Effect on Cell . METHODS: A total of 58 gastrectomy specimens were performed for TUNEL and immunohistochemical staining for Bcl 2, Bcl XL, Bcl XS/L, BAX and Mcl 1 proteins. Results The apoptotic index of high-grade and moderately-differentiated adenocarcinomas was significantly higher than that of poorly-differentiated and undifferentiated adenocarcinomas (P < 0.05). The apoptosis index of gastric cancer tissues with positive expression of Bcl 2, Bcl XL, and Mcl 1 antigens was significant. The apoptotic index of gastric cancer tissues with positive expression of BAX and Bcl XS/L antigen was significantly lower than that of gastric cancer tissues with negative expression of two antigens (P < 0.01). The expression of Bcl 2, Bcl XL, BAX, and Bcl XS/L in high- and middle-differentiated adenocarcinomas was significantly higher than that of poorly differentiated adenocarcinomas and undifferentiated adenocarcinomas (P < 0.01). The expression of Mcl 1 in poorly differentiated adenocarcinoma was significantly higher than that in high and moderately differentiated adenocarcinoma (P < 0.05). Conclusion High and moderately differentiated adenocarcinomas have high apoptosis rate, and pro-apoptotic gene positive gastric cancer tissues have high apoptosis rate. Bcl 2 , Bcl XL, BAX, and Bcl XS/L may be involved in the development of intestinal type gastric cancer, whereas Mcl 1 mainly affects diffuse type gastric cancer.