论文部分内容阅读
目的探讨多瘤抑癌基因蛋白p16表达与原发性和继发性头颈部肿瘤生物学行为之间的关系。方法通过细针吸取及术中印片细胞学诊断病例获取标本,进行免疫细胞化学技术分析,检测220例头颈部良、恶性肿瘤及其他病变中p16蛋白表达水平,并对头颈部肿瘤组织学类型、分化程度的关系进行分析研究。结果 p16蛋白在头颈部良性病变的阳性表达率为84.63%,明显高于恶性肿瘤的表达率53.90%,差异有显著性(P<0.05)。p16蛋白表达与恶性肿瘤的细胞分化程度有关,高分化肿瘤其表达水平明显高于低分化肿瘤,同时显示p16蛋白表达程度与头颈部肿瘤大小及有无转移均无明显关联。结论多瘤抑癌基因蛋白p16参与了肿瘤的发生及分化,检测p16蛋白表达水平,可能成为肿瘤诊断和预测预后的一项新指标。
Objective To investigate the relationship between the expression of multi-tumor suppressor gene protein p16 and the biological behaviors of primary and secondary head and neck tumors. Methods Fine needle aspiration and intraoperative printing cytology were used to obtain samples. Immunocytochemistry was used to detect the expression of p16 protein in 220 cases of head and neck benign and malignant tumors and other pathological changes. Tumor histology Type, degree of differentiation of the relationship between analysis and research. Results The positive expression rate of p16 protein in benign lesions of head and neck was 84.63%, which was significantly higher than that of malignant tumors (53.90%) (P <0.05). The expression of p16 protein was related to the degree of cell differentiation in malignant tumors. The expression of p16 protein in well differentiated tumors was significantly higher than that in poorly differentiated tumors. There was no significant correlation between the expression of p16 protein and tumor size and metastasis in head and neck. Conclusions Multi-tumor anti-oncogene protein p16 is involved in tumorigenesis and differentiation. Detection of p16 protein expression may be a new indicator of tumor diagnosis and prognosis.