目的:在三叉神经主核(ＰｒＶ)上检测不同剂量甘丙肽拮抗剂Ｍ３５的中枢效应。方法:运用全细胞膜片钳技术。结果:运用Ｍ３５ ０．０１μｍｏｌ·Ｌ－１、０．０３μｍｏｌ·Ｌ－１及０．１μｍｏｌ·Ｌ－１时,甘丙肽引发的外向性电流分别抑制 ３２．８%、７０．７%及７７．６%,Ｍ３５１ μｍｏｌ·Ｌ－１及３ μｍｏｌ·Ｌ－１使甘丙肽外向性电流分别增大 ２８．４%及 ４９．１%。结论:Ｍ３５低浓度为甘丙肽拮抗剂,Ｍ３５高浓度有甘丙肽激动剂作用,ＰｒＶ同一神经元上可能存在不同甘丙肽亚型受体。 PURPOSE: To examine the central effects of different doses of the galanin antagonist M35 on the main trigeminal nerve (PrV). Methods: Whole-cell patch clamp technique was used. Results: With M35 0.01μmol·L-1, 0.03μmol·L-1 and 0.1μmol·L-1, the outward current induced by galanin was inhibited by 32.8%, 70.7% and 77%, respectively .6%, M351 μmol·L-1 and 3 μmol·L-1 increased the outward current of galanin by 28.4% and 49.1%, respectively. Conclusion: M35 is a low-dose galanin antagonist with a high concentration of M35 and a galanin agonist. There may be different galanin subtype receptors on the same neuron of PrV.