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以羟脯氨酸（ＨＹＰ）为选择剂，通过连续多步筛选法和间隙多步筛选法，从二倍体葡萄里扎马特（Ｒｉｚａｍａｔ）品种的能再生愈伤组织中，分离出了７个稳定的ＨＹＰ抗性变异细胞无性系，连续多步选择法的选择效率为４．２４×１０－５，间隙多步选择法的选择效率为７．００×１０－５．但连续多步选择法更有利于高脯氨酸累积变异系的选择。７个ＨＹＰ抗性变异系都能稳定的累积脯氨酸，其游离脯氨酸的含量是对照的１．７９～３．９３倍。
Hydroxyproline (HYP) was used as a selective agent to separate callus from regenerated callus of diploid grape Rizamat by continuous multistep selection and multistep selection. A stable HYP resistant mutant clones, the selection efficiency of continuous multistep selection was 4.24 × 10-5, and the selection efficiency of gap multistep selection was 7.00 × 10-5. However, the continuous multi-step selection method is more conducive to the selection of high proline mutant lines. All 7 HYP resistant lines could accumulate proline steadily with free proline content of 1.79-3.93 times that of the control.