论文部分内容阅读
[目的]探讨p53Arg72Pro多态性与HPV相关宫颈癌发生机制的关系。[方法]采用PCR技术检测210例宫颈癌和95例正常宫颈组织的HPV16DNA,采用免疫组化方法及TUNEL检测p53Arg72Pro三种基因型中p53、p21、Bax、Ki-67蛋白(PI)表达以及细胞凋亡(AI)。[结果]宫颈癌HPV16阳性率为70.5%,与正常宫颈组织(7.4%)相比差异具有统计学意义(P<0.05)。HPV16阳性的宫颈癌中:①p53蛋白阴性和弱阳性表达率(73.6%)高于强阳性率(26.4%),其中p53Arg的阴性表达率(39.2%)高于p53Pro(16.7%),差异有统计学意义(P<0.05);②p21蛋白阴性和弱阳性组中,p53Pro型中PI高于p53Arg型,差异有统计学意义(P<0.05);③Bax蛋白阴性和弱阳性组中,p53Pro型中AI低于p53Arg型,差异有统计学意义(P<0.05)。[结论]p53蛋白可被HPV16E6蛋白降解,其中p53Arg蛋白更易被降解;p53Arg和p53Pro蛋白被降解后,两者抑制细胞增殖能力的降低和诱导细胞凋亡能力的降低程度不同,其中p53Pro蛋白转录激活p21和Bax基因的功能及细胞周期阻滞作用的降低更明显。
[Objective] To investigate the relationship between p53Arg72Pro polymorphism and HPV-related cervical carcinogenesis. [Methods] The HPV16 DNA of 210 cases of cervical cancer and 95 cases of normal cervical tissue were detected by PCR. The expressions of p53, p21, Bax and Ki-67 protein (PI) and p53 Apoptosis (AI). [Results] The positive rate of HPV16 in cervical cancer was 70.5%, which was significantly different from that in normal cervical tissue (7.4%) (P <0.05). HPV16-positive cervical cancer: ①p53 protein negative and weak positive expression rate (73.6%) higher than the strong positive rate (26.4%), which p53Arg negative expression rate (39.2%) higher than that of p53Pro (16.7%), the difference was statistically (P <0.05). ② In p21 protein negative and weakly positive groups, the PI of p53Pro was higher than that of p53Arg (P <0.05); ③ In the negative and weak positive groups of p53, Lower than p53Arg type, the difference was statistically significant (P <0.05). [Conclusion] p53 protein can be degraded by HPV16E6 protein, of which p53Arg protein is more likely to be degraded. After p53Arg and p53Pro protein are degraded, both the reduction of cell proliferation ability and the decrease of apoptosis inducing capacity are different. The transcriptional activation of p53Pro protein The p21 and Bax gene function and cell cycle arrest more significantly reduced.