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目前已有许多关于输血后非甲非乙型肝炎(NANB)病毒的分离以及相关抗原抗体系统方面的报告,但大多未能得到大家的一致承认,所以人们很快也就忘却了。这次作者从非甲非乙型肝炎感染的黑猩猩血浆中,成功地分离出一种很有意义的病毒,现报告如下: 材料和方法:材料采用NANB(来自NANB患者血清的NIH-F株)感染的黑猩猩(3例)慢性期血浆(各500ml),将其浓缩约1000倍,最后通过蔗糖密度梯度平衡离心进行精制分离。对照组是1例非感染的黑猩猩血浆,采用相间方法处理。结果:在蔗糖密度为1.14～1.48g/ml(峰值=1.16)的各部分中,用负染法制作的电镜观察,发现有胞膜鲜明的球状病毒颗粒(直径为37～64nm)。从形态学看,这种病毒类似toga病毒。另外在肝细胞中
There have been many reports on the isolation of non-A, non-B hepatitis B (NANB) virus and related antigen-antibody systems after transfusion. However, most of them failed to receive the unanimous acknowledgment of everyone and people quickly forgot to do so. The authors successfully isolated from non-A, non-Hepatitis B infected chimpanzee plasma, a meaningful virus, are reported as follows: Materials and Methods: Materials NANB (from NANB patient serum NIH-F strain) Infected chimpanzees (3 cases) with chronic phase plasma (500 ml each) were concentrated about 1000-fold and finally purified by centrifugation with sucrose density gradient centrifugation. The control group, a noninfected chimpanzee plasma, was treated with the interphase method. Results: Observed by electron microscopy using a negative staining method, a clear membrane-like globular virus particle (37-64 nm in diameter) was found in each fraction with a sucrose density of 1.14-1.48 g / ml (peak = 1.16). Morphologically, this virus is similar to the toga virus. In addition in the liver cells