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目的:考察海藻酸钠对硝苯地平大鼠肠吸收的影响以及其对大鼠P-gp表达的影响。方法:运用大鼠全小肠单向灌流模型研究海藻酸钠对硝苯地平大鼠肠渗透性的影响,并采用HPLC法测定肠灌流液中硝苯地平的浓度;采用western blot技术研究海藻酸钠对大鼠小肠P-gp表达的影响。结果:海藻酸钠的浓度达到一定值后,会提高硝苯地平的小肠有效渗透系数;硝苯地平的自身浓度对肠吸收有影响,高浓度硝苯地平的小肠有效渗透系数略有提高;海藻酸钠相对分子质量的变化和灌流液p H值的变化对硝苯地平的小肠有效渗透系数无显著性影响;连续给予海藻酸钠和维拉帕米(P-gp抑制剂)后,P-gp的表达量增加。结论:海藻酸钠能促进硝苯地平的大鼠肠吸收。
Objective: To investigate the effect of sodium alginate on intestinal absorption of nifedipine and its effect on P-gp expression in rats. Methods: The intestine permeability of nifedipine rats was studied by rat intestine unidirectional perfusion model. The concentration of nifedipine in intestinal perfusate was determined by HPLC. The effects of sodium alginate On intestinal P-gp expression in rats. Results: After the concentration of sodium alginate reached a certain value, the effective permeability coefficient of nifedipine in small intestine was increased. The self-concentration of nifedipine had an effect on intestinal absorption, and the effective osmotic coefficient of small intestine of nifedipine increased slightly. The change of relative molecular mass of sodium and sodium and the change of p H of perfusate had no significant effect on the effective permeability coefficient of nifedipine. After continuous administration of sodium alginate and verapamil (P-gp inhibitor), P- The expression of gp is increased. Conclusion: Sodium alginate can promote intestinal absorption of nifedipine in rats.