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建立了以三联吡啶钌为发光体系的毛细管电泳电化学发光(CE-ECL)检测系统,并应用于分离和测定西咪替丁片剂中西咪替丁的含量。考察了检测电位,三联吡啶钌(Ru(bpy)32+)的溶液浓度,缓冲液的pH和溶液浓度,分离电压、进样电压与进样时间等因素对分离检测的影响。结果表明:在检测电位1.18V,Ru(bpy)32+溶液浓度为5 mmol/L,磷酸盐缓冲液(PBS)25 mmol/L(pH 7.8),进样时间10 s,进样电位10 kV,运行电位15 kV下,测得西咪替丁线性范围为2.8×10-6~4.0×10-4mol/L,检出限为1.2×10-7mol/L(S/N=3)。对1.0×10-5mol/L的西咪替丁标准溶液连续测定5次,电化学发光强度和迁移时间的RSD分别为3.9%和1.5%。方法已应用于西咪替丁片剂中西咪替丁含量的测定。
A capillary electrophoresis electrochemiluminescence (CE-ECL) detection system based on ruthenium bipyridyl was established and applied to the separation and determination of cimetidine in cimetidine tablets. The influences of the detection potential, the concentration of Ru (bpy) 32 (Ru (bpy) 32+), the pH value of the buffer solution and the concentration of the solution, the separation voltage, the injection voltage and the injection time on the separation and detection were investigated. The results showed that when the detection potential was 1.18V, the concentration of Ru (bpy) 32+ solution was 5 mmol / L, the phosphate buffer solution was 25 mmol / L (pH 7.8), the injection time was 10 s and the injection potential was 10 kV , The linear range of cimetidine was 2.8 × 10-6 ~ 4.0 × 10-4mol / L and the detection limit was 1.2 × 10-7mol / L (S / N = 3) at the operating potential of 15 kV. The RSD of electrochemiluminescence intensity and migration time were 3.9% and 1.5% respectively after continuously measuring 5 times the standard solution of cimetidine 1.0 × 10-5mol / L. The method has been applied to the determination of cimetidine in cimetidine tablets.