论文部分内容阅读
目的:探讨结直肠癌中突变型P53基因(mP53)和增殖细胞核抗原(PCNA)表达的相关性及临床意义。方法:应用免疫组化二步法,检测60例结直肠癌组织及20例正常肠粘膜中mP53、PCNA的表达,结合临床病理资料进行统计分析。结果:60例结直肠癌中mP53阳性表达率65.0%,PCNA阳性表达率78.3%,20例正常肠粘膜中mP53、PCNA表达均为阴性(P<0.05)。mP53和PCNA阳性表达率在低分化组、浆膜层浸润组、淋巴结转移组均较高(P均<0.05)。mP53和PCNA表达呈正相关(r=0.58,P<0.05)。结论:mP53和PCNA在结直肠癌中表达均增高,二者与结直肠癌病理学分级、浸润深度和淋巴结转移有关,可作为判断结直肠癌预后的参考指标。
Objective: To investigate the correlation and clinical significance of the expression of mutant p53 gene and proliferating cell nuclear antigen (PCNA) in colorectal cancer. Methods: The expression of mP53 and PCNA in 60 cases of colorectal cancer tissues and 20 cases of normal intestinal mucosa were detected by immunohistochemical two-step method. Statistical analysis was made based on the clinicopathological data. Results: The positive expression rates of mP53 and PCNA in 60 cases of colorectal cancer were 65.0% and 78.3%, respectively. The expression of mP53 and PCNA in 20 cases of normal intestinal mucosa were all negative (P <0.05). The positive rates of mP53 and PCNA were higher in poorly differentiated group, serosal invasion group and lymph node metastasis group (all P <0.05). There was a positive correlation between mP53 and PCNA expression (r = 0.58, P <0.05). Conclusion: The expressions of mP53 and PCNA in colorectal cancer are both increased, which may be related to the pathological grade, depth of invasion and lymph node metastasis of colorectal cancer, which may serve as a reference index for prognosis of colorectal cancer.