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目的:比较自身免疫性肝炎(autoimmune hepatitis,AIH)患者与健康对照者(healthy controls,HCs)外周血CD4+CD25+调节性T细胞(CD4+CD25+Tregs)数量、免疫抑制功能的变化,探讨CD4+CD25+Tregs参与AIH发病的可能机制。方法:采用流式细胞仪检测8例AIH患者及15例健康对照组的外周血CD4+CD25+Tregs数量的百分比及绝对数量;采用共同培养方法检测AIH患者外周血CD4+CD25+Tregs的免疫抑制功能的变化;实时荧光定量聚合酶链反应(RT-FQ-PCR)检测AIH患者外周血CD4+CD25+Tregs中FoxP3mRNA的表达。结果:AIH患者外周血CD4+CD25+Tregs数量明显低于HCs(p<0.01);混合淋巴细胞共同培养结果显示,AIH患者外周血CD4+CD25+Tregs抑制功能明显低于HCs组(p<0.01);AIH患者外周血CD4+CD25+Tregs的FoxP3mRNA相对表达量显著降低,与HCs组比较有显著性差异(p<0.01)。结论:CD4+CD25+Tregs细胞的数量的减少和Foxp3表达的降低所造成的CD4+CD25+Tregs细胞免疫抑制功能受损可能是AIH发病的一个因素。
Objective: To compare the changes of CD4 + CD25 + regulatory T cells (CD4 + CD25 + Tregs) and immunosuppressive function in peripheral blood of patients with autoimmune hepatitis (AIH) and healthy controls (HCs) + CD25 + Tregs participate in the pathogenesis of AIH. Methods: The percentage and absolute number of CD4 + CD25 + Tregs in peripheral blood of 8 AIH patients and 15 healthy controls were detected by flow cytometry. The co-culture method was used to detect the immunosuppression of CD4 + CD25 + Tregs in peripheral blood of AIH patients The changes of FoxP3 mRNA in CD4 + CD25 + Tregs in peripheral blood of AIH patients were detected by real-time fluorescent quantitative polymerase chain reaction (RT-FQ-PCR). Results: The number of CD4 + CD25 + Tregs in peripheral blood of AIH patients was significantly lower than that of HCs (p <0.01). The co-culture of mixed lymphocytes showed that the inhibitory function of CD4 + CD25 + Tregs in peripheral blood of AIH patients was significantly lower than that of HCs group ). The relative expression of FoxP3mRNA in CD4 + CD25 + Tregs of AIH patients was significantly lower than that of HCs group (p <0.01). Conclusion: The decreased immunosuppressive function of CD4 + CD25 + Tregs cells induced by the decrease of CD4 + CD25 + Tregs cells and the decrease of Foxp3 expression may be one of the pathogenesis of AIH.