HPLC法测定瓦松栓中槲皮素和山柰素的含量

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目的建立一种高效液相色谱法测定瓦松栓中槲皮素和山柰素含量的方法。方法采用高效液相色谱法,用十八烷基硅烷键合硅胶为填充剂;流动相A为乙腈,流动相B为1.0%盐酸溶液;检测波长:360nm。结果在该色谱条件下,槲皮素、山柰素的线性范围分剐为0。006~0.024mg·mL-1(r=0.9908)、0.012~0.047mg·mL-1(r=0.9952),平均回收率分别为99.91%、101.2%。结论该方法简单准确,重现性好,可作为瓦松栓中槲皮素和山柰素的含量测定方法。
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