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目的研究内蒙古地区蒙、汉族人群谷胱甘肽硫转移酶M1基因(GSTM1)多态性,探讨蒙古族人群肺癌易感性与GSTM1基因多态性关系。方法采用多重PCR等方法对325名健康汉族人、310名健康蒙古族人及304例蒙古族肺癌患者进行GSTM1基因多态性检测,分析GSTM1基因型的分布频率,并探讨GSTM1基因多态性与蒙古族人群肺癌易感性的关系。结果健康蒙、汉族人群GSTM1基因型分布频率分别为:GSTM1功能型(+)61.61%,GSTM1缺陷型(-)38.39%和GSTM1(+)57.23%,GSTM1(-)42.77%;蒙古族肺癌组与对照组GSTM1(+)和GSTM1(-)的分布频率分别为52.96%,47.04%和61.61%,38.39%;携带GSTM1(-)的个体患肺癌的风险增高,OR值为1.43(95%CI=1.03~1.96,P<0.05)。结论GSTM1基因型分布频率在内蒙古地区蒙、汉族人群间比较无明显差别;GSTM1(-)是蒙古族人群肺癌的易感因素。
Objective To investigate the polymorphism of glutathione S-transferase M1 gene (GSTM1) in Mongolian and Han nationality in Inner Mongolia, and explore the relationship between lung cancer susceptibility and GSTM1 gene polymorphism in Mongolian population. Methods The polymorphisms of GSTM1 gene in 325 healthy Han, 310 healthy Mongolian and 304 Mongolian patients with lung cancer were detected by multiplex PCR, the distribution frequency of GSTM1 genotypes was analyzed, and the relationship between GSTM1 gene polymorphism and Mongolia Relationship between Susceptibility to Lung Cancer in Ethnic Groups. Results The frequencies of GSTM1 genotype in healthy Mongolian and Han nationality were 61.61% for GSTM1, 38.39% for GSTM1 deficiency (-), 57.23% for GSTM1 (+) and 42.77% for GSTM1 (-), The distribution frequency of GSTM1 (+) and GSTM1 (-) in control group was 52.96%, 47.04% and 61.61%, 38.39% respectively. The risk of lung cancer was higher in individuals with GSTM1 (-), with an odds ratio of 1.43 = 1.03 ~ 1.96, P <0.05). Conclusion There is no significant difference in the frequency of GSTM1 genotype between Mongolian and Han nationalities in Inner Mongolia. GSTM1 (-) is a predisposing factor for lung cancer in Mongolian population.