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目的探索国人Ｗｉｌｓｏｎ病（ＷＤ）基因位点（ＷＮＤ）与ＡＦＭ２３８ｖｃ３、Ｄ１３Ｓ３０１、Ｄ１３Ｓ３１６、Ｄ１３Ｓ２９６、及ＡＦＭ０８４ｘｃ５等５个微卫星ＤＮＡ（ＳＴＲ）之连锁关系，对ＷＮＤ进行精确定位。为ＷＤ基因克隆奠定基础。方法对２０个ＷＤ家系１１３名成员及１００名正常对照组成员的ＤＮＡ进行５个ＳＴＲ的聚合酶链反应（ＰＣＲ）扩增片段长度多态性分析；对ＷＮＤ及５个ＳＴＲ进行二点配对及多点连锁分析。结果ＷＮＤ与Ｄ１３Ｓ３０１、Ｄ１３Ｓ３１６、Ｄ１３Ｓ２９６呈紧密连锁，与ＡＦＭ２３８ｖｃ３及ＡＦＭ０８４ｘｃ５呈中度连锁。结论ＷＮＤ与５个ＳＴＲ的遗传连锁图谱为：着丝点—ＡＦＭ２３８ｖｃ３—Ｄ１３Ｓ３０１—ＷＮＤ—Ｄ１３Ｓ３１６—Ｄ１３Ｓ２９６—ＡＦＭ０８４ｘｃ５—端粒
Objective To explore the linkage between Wilson ’s disease (WD) loci (WND) and five microsatellite DNAs (STR) such as AFM238vc3, D13S301, D13S316, D13S296, and AFM084xc5 and to locate WND accurately. Lay the foundation for the cloning of WD gene. Methods Five STRs were amplified by polymerase chain reaction (PCR) amplification of DNA from 113 members of 20 WD families and 100 normal control subjects. Two pairs of WND and five STRs Multi-point chain analysis. Results WND was closely linked with D13S301, D13S316 and D13S296, and moderately linked with AFM238vc3 and AFM084xc5. Conclusion The genetic linkage map between WND and five STRs is centromere-AFM238vc3-D13S301-WND-D13S316-D13S296-AFM084xc5-telomere