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目的了解我国部分地区新型肝炎病毒ＴＴＶ感染的分子流行病学、ＴＴＶ在人群中的感染分布状况，探讨ＴＴＶ在肝炎发病中的作用和地位。方法采用ＰＣＲ方法检测血清标本中ＴＴＶＤＮＡ，克隆测定不同地理株ＴＴＶ的部分基因序列，分析其基因变异情况。结果检测了南方（广东深圳和江苏南京）和北方（北京和辽宁沈阳）的１１２例非甲～非庚型肝炎病人，其中ＴＴＶＤＮＡ阳性者４８例，阳性率４２．９％，而１０２例甲～庚型肝炎病人中阳性率为２．９％（χ２＝４２．８，Ｐ＜０．０１）。ＡＬＴ异常而无甲～庚型肝炎病毒感染标志的献血员中，ＴＴＶＤＮＡ阳性率（３４６％）明显高于ＡＬＴ正常献血员的阳性率（１６．８％，χ２＝４．５，Ｐ＜０．０１）。南方株（广东深圳ＴＴＶＣＨＮ００２、南京ＴＴＶＮＡＮ００１）和北方株（北京ＴＴＶＳＨＢ０１５）病毒间同源性在９８％以上，日本发表的序列同源性也高于９７％。结论我国北方和南方地区均存在ＴＴＶ感染。ＴＴＶ感染与ＡＬＴ异常有极为密切的关系，可能是导致非甲～非庚型肝炎的重要病原。正常人群中存在ＴＴＶ感染者，类似于ＨＢｓＡｇ的所谓“慢性携带状态”。
Objective To understand the molecular epidemiology of novel hepatitis virus TTV infection in some parts of China and the distribution of infection in the population of TTV and to explore the role and status of TTV in the pathogenesis of hepatitis. Methods TTVDNA of serum samples was detected by PCR, and some gene sequences of TTV from different geographical strains were cloned and analyzed. Results A total of 112 cases of non-A to non-G hepatitis were detected in the south (Shenzhen and Nanjing, Jiangsu) and in the north (Beijing and Shenyang, Liaoning). Of the 48 cases, 48 were positive for TTVDNA and the positive rate was 42.9% The positive rate of hepatitis G patients was 2.9% (χ2 = 42.8, P <0.01). The positive rate of TTVDNA (346%) was significantly higher than that of ALT normal blood donors (16.8%, χ2 = 4.5, P < 0.01). The homology between South China (Shenzhen TTVCHN002, Nanjing TTVNAN001) and North China (Beijing TTVSHB015) viruses is over 98%, and the sequence homology published in Japan is higher than 97%. Conclusion TTV infection exists in both northern and southern China. TTV infection and abnormal ALT have a very close relationship may be caused by non-A to non-hepatitis G, an important pathogen. TTV infection exists in the normal population, similar to the so-called “chronic carrier status” of HBsAg.