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目的探讨羽扇豆醇对小鼠异基因骨髓移植(allo-BMT)后急性移植物抗宿主病(aGVHD)的预防作用。方法 25只小鼠随机均分为羽扇豆醇1mg/只高剂量组(A1组)、羽扇豆醇0.5mg/只中剂量组(A2组)、羽扇豆醇0.25mg/只低剂量组(A3组)、模型对照组(B组)和正常对照组(C组)。采用减弱强度的预处理方式建立aGVHD动物模型,HE染色观察aGVHD小鼠的病理改变,ELISA法检测血清炎性细胞因子的水平变化。结果 A1组小鼠中位生存期明显长于B组(27dvs.17d)(P<0.05);与B组相比,A1组aGVHD病理改变减轻,炎性细胞因子水平降低(P<0.05),而A2、A3组各观察指标差异无统计学意义。结论高剂量羽扇豆醇可显著延长aGVHD小鼠生存时间,有效减轻aGVHD病理损害程度。
Objective To investigate the preventive effect of lupeol on acute graft-versus-host disease (aGVHD) after allogeneic bone marrow transplantation (allo-BMT) in mice. Methods Twenty-five mice were randomly divided into 1 group (group A1), 0.5 mg / l lucenol group (group A2) and 0.25 mg / l lumenol group (group A3) Group), model control group (group B) and normal control group (group C). The animal models of aGVHD were established by attenuated preconditioning. The pathological changes of aGVHD mice were observed by HE staining. The levels of serum inflammatory cytokines were measured by ELISA. Results The median survival time in group A1 was significantly longer than that in group B (27d vs. 17d) (P <0.05). Compared with group B, the pathological changes of aGVHD and the levels of inflammatory cytokines in group A1 were decreased (P <0.05) There was no significant difference between the two groups in the observation of A2 and A3 groups. Conclusion High dose of lupine alcohol can significantly prolong the survival time of aGVHD mice and effectively reduce the pathological damage of aGVHD.