玻璃离子粘固粉在牙科修复领域已得到广泛应用。多项研究表明,它可释放氟成分至水性环境中,减少继发龋的发生,但氟对牙髓细胞是否有毒性作用仍不清楚,本文对此进行了研究。 材料和方法 因正畸需要拔除的人类第二前磨牙,取出牙髓后采用标准移植术进行培养,至第三和第八时期时,用于氟对离体牙髓细胞的影响研究。项目包括H33258荧光测定、细胞增生、蛋白合成及线粒体活性测定。 结果与讨论 氟使牙髓细胞密度减少,并在24 Glass ionomer cement has been widely used in the field of dental restoration. A number of studies have shown that it can release fluorine components to the aqueous environment and reduce the occurrence of secondary caries, but the role of fluorine on dental pulp cells is still not clear, this article was studied. MATERIALS AND METHODS: Human second premolar human tooth extracted for orthodontic treatment, dental pulp removed after standard transplantation for culture, to the third and eighth period for fluorine on isolated dental pulp cells. The project includes H33258 fluorescence assay, cell proliferation, protein synthesis and mitochondrial activity measurement. RESULTS AND DISCUSSION Fluoride reduced the density of dental pulp cells, and at 24