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目的:找到一种简单、快速、准确的进行病毒性腹泻患者粪便标本中诺如病毒的检测方法,并对此方法进行评价。方法:用恒温快速检测方法进行154例北京地区病毒性腹泻患者粪便标本诺如病毒的检测,同时用逆转录-聚合酶链反应(RT-PCR)作对照,评价该方法。结果:在154例病毒性腹泻的病人标本中,采用恒温快速检测方法进行诺如病毒检测,阳性结果61例,阳性率为39.6%,RT-PCR方法进行检测,阳性结果54例,阳性率为35.1%,以RT-PCR为金标准计算,检测灵敏度90.7%(49/54),特异性88%(88/100)。这两组检验结果经过2χ检验,P=0.143,P值>0.05,说明两组结果之间差异无统计学意义,即两种实验方法结果无差异。通过一致性检验显示,KAPPA=0.765,P=0.000<0.05,说明这两种试验方法结果一致性较好。结论:恒温快速检测方法是一种简单、快速、准确的检测诺如病毒的方法,适合临床及流行病学筛查使用。
OBJECTIVE: To find out a simple, rapid and accurate method for detection of norovirus in stool samples of patients with viral diarrhea and evaluate the method. Methods: The detection of norovirus in 154 cases of viral diarrhea in Beijing was carried out by the constant temperature and rapid detection method, and the method was evaluated by reverse transcription - polymerase chain reaction (RT - PCR). Results: In 154 patients with viral diarrhea, novirus was detected by the rapid thermostatic assay. The positive results were 61 cases (39.6%). RT-PCR was used to detect the positive results in 54 cases. The positive rate was 35.1%. The detection sensitivity was 90.7% (49/54) and 88% (88/100) based on the standard of RT-PCR. 2χ test results of these two groups of tests, P = 0.143, P value> 0.05, indicating no significant difference between the two groups of results, that is, there is no difference between the two experimental methods. Through the consistency test showed that KAPPA = 0.765, P = 0.000 <0.05, indicating that the two test methods better consistency. Conclusion: The rapid thermostat detection method is a simple, rapid and accurate method for detecting Norovirus, suitable for clinical and epidemiological screening.