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目的 阐明微量元素Se、Cr含量异常时肝细胞损害的机理。方法 用细胞内微电极技术分别观察了 :正常对照 (两组 )、单纯低Se、低Se高维生素C、单纯低Cr、低Cr高维生素C、低Se低Cr、低Se低Cr伴高维生素C饲料饲养 14w后大鼠肝细胞电生理的改变。结果 单纯低铬组无变化 ;单纯低硒组RP、APA降低 ,APD50 、APD90延长 ;低硒并低铬组RP、APA降低 ,APD50 、APD90 明显延长 ;低Se高维生素C组仅RP降低 ,APA、APD与对照组相比差异无显著性意义。结论 低硒并低铬可明显改变肝细胞的电生理特性 ,维生素C可以对抗低Se并低Cr所致的这种变化
Objective To elucidate the mechanism of liver cell damage in the presence of trace elements Se and Cr. Methods The intracellular microelectrode technique was used to observe the effects of low-Se, low-se vitamin C, low-Cr, low-Cr high vitamin C, low-Se low Cr, low-Se low Cr with high vitamin Electrophysiological Changes of Rat Hepatocytes after 14 Days of Feeding. Results Low and low selenium groups showed no change in RP and APA but APD50 and APD90 prolonged. RP and APA in low selenium and low chromium group decreased and APD50 and APD90 were significantly prolonged. In low Se and high vitamin C group, only RP and APA There was no significant difference between APD and control group. Conclusion Low selenium and low chromium can obviously change the electrophysiological characteristics of hepatocytes. Vitamin C can antagonize this kind of change with low Se and low Cr