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为了进一步阐明肿瘤疫苗的特异性主动免疫治疗机理,采用β-榄香烯、丝裂霉素C(MMC)及热休克在体外单独及复合处理小鼠H22肝癌细胞,经间接免疫荧光染色,流式细胞仪分析,结果显示.上述处理因素均能明显地诱导H22细胞表达HSP70(以阳性率增高表示).在单因素中,以β-榄香烯效果最佳,阳性率为69.16%,MMC次之;在复合因素中.以β-榄香烯与MMC复合处理的效果最佳,阳性率为95.13%;三种因素联合处理的效果为85.34%.提示瘤苗的抗肿瘤免疫作用是与HSP70表达程度有关.
To further elucidate the mechanism of specific active immunotherapy for tumor vaccines, mouse H22 hepatoma cells were treated with β-elemene, mitomycin C (MMC) and heat shock in vitro and in vitro, and indirect immunofluorescence staining was performed. By cytometric analysis, the results showed that the above treatment factors can obviously induce H22 expression in H22 cells (expressed as a positive rate). Among the single factors, β-elemene was the best, the positive rate was 69.16%, MMC. Secondly, among the composite factors, the combination of β-elemene and MMC had the best effect, the positive rate was 95.13%, and the combined effect of the three factors was 85.34%. It was suggested that the antitumor immunity of tumor vaccine was The degree of HSP70 expression is related.