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目的:通过β受体阻滞剂普萘洛尔作用于小鼠EOMA血管瘤细胞体外增殖及凋亡的实验研究,初步探讨普萘洛尔治疗血管瘤的机制。方法:体外培养EOMA细胞,使用不同浓度普萘洛尔分别作用于EOMA细胞24、36、48 h,应用噻唑蓝(MTT)法检测细胞存活率、吖啶橙染色检测细胞凋亡情况,观察普萘洛尔对EOMA细胞体外增殖和凋亡的影响。结果:作用24 h后,随剂量增加,EOMA细胞存活率逐渐下降,与对照组相比,至药物浓度为75μmol/L时有显著差异(P<0.05),继续增加至800μmol/L时,细胞存活率接近0,同时吖啶橙染色示凋亡细胞逐渐增多,与对照组相比,至药物浓度75μmol/L时,细胞凋亡率有显著差异(P<0.05)。36 h组和48 h组变化趋势与24h组相似。结论:普萘洛尔在体外可有效抑制小鼠EOMA血管瘤细胞的增殖并促进其凋亡,这一作用呈现明显的剂量-时间效应依赖性。
OBJECTIVE: To explore the mechanism of propranolol in the treatment of hemangiomas through the effect of propranolol, a beta-blocker, on proliferation and apoptosis of mouse EOMA hemangio cells in vitro. Methods: EOMA cells were cultured in vitro. Propranolol was administered to EOMA cells in different concentrations for 24, 36 and 48 h respectively. Cell viability was detected by MTT assay. Apoptosis was detected by acridine orange staining. Effects of Nalolol on Proliferation and Apoptosis of EOMA Cells in. Results: The survival rate of EOMA cells decreased gradually with the increase of dose at 24 h. Compared with the control group, there was a significant difference (P <0.05) when the drug concentration was 75 μmol / L, and continued to increase to 800 μmol / L. The survival rate was close to 0, while acridine orange staining showed apoptotic cells gradually increased compared with the control group, to the drug concentration 75μmol / L, the apoptosis rate was significantly different (P <0.05). 36 h and 48 h group trends and 24h group similar. CONCLUSIONS: Propranolol can effectively inhibit the proliferation and promote the apoptosis of EOMA hemangio cells in vitro in vitro, and this effect shows obvious dose-time dependence.