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为了探讨川芎嗪对心肌缺血 再灌注损伤 (MIRI)时心肌细胞线粒体功能及结构的影响。本实验选用日本大耳白兔 30只 ,随机分为正常对照组 (A组 )、心肌缺血 再灌注损伤组 (B组 )和心肌缺血 再灌注损伤 +川芎嗪治疗组(C组 )。复制MIRI模型 ,分别观察心肌线粒体呼吸功能、Ca2 + 浓度 ([Ca2 + ]m)、丙二醛浓度 (MDA)、超氧化物歧化酶活性 (SOD)及超微结构的改变和心肌组织三磷酸腺苷 (ATP)、二磷酸腺苷 (ADP)、一磷酸腺苷 (AMP)含量、总腺苷酸量 (TAN)、能荷 (EC)的变化。结果发现 ,C组与B组比较 ,线粒体呼吸控制率 (RCR)、Ⅲ态呼吸速率 (ST3)、SOD、面密度 (Sv)、比表面 (δ)明显升高 ,Ⅳ态呼吸速率 (ST4 )、[Ca2 + ]m 、MDA、体密度 (Vv)、横径 (Hd)显著降低 ,心肌组织ATP、ADP、TNA及EC均明显增高 ;且与A组比较 ,ST3、ST4 、SOD、Vv、Sv、δ、数密度 (Nv)、纵径 (Vd)及ADP、AMP、TNA无明显差异。可见 ,川芎嗪可通过降低氧自由基水平和减轻钙超载 ,而改善缺血 再灌注损伤心肌的线粒体功能及结构。
To investigate the effect of tetramethylpyrazine on myocardial mitochondrial function and structure during myocardial ischemia-reperfusion injury (MIRI). In this experiment, 30 Japanese white rabbits were randomly divided into normal control group (A group), myocardial ischemia-reperfusion injury group (B group), and myocardial ischemia-reperfusion injury + ligustrazine treatment group (C group). The MIRI model was duplicated and myocardial mitochondrial respiratory function, Ca2+ concentration ([Ca2+]m), malondialdehyde (MDA), superoxide dismutase activity (SOD) and ultrastructure changes, and myocardial tissue adenosine triphosphate (ATP) were observed. ATP), adenosine diphosphate (ADP), adenosine monophosphate (AMP) content, total adenine nucleotide (TAN), energy load (EC) changes. The results showed that the mitochondrial respiratory control rate (RCR), III state respiratory rate (ST3), SOD, areal density (Sv), specific surface (δ) were significantly higher in the C group than in the B group, and the IV state respiration rate (ST4). Calcium, [Ca2 + ]m, MDA, body density (Vv), and transverse diameter (Hd) decreased significantly, and myocardial ATP, ADP, TNA, and EC increased significantly. Compared with group A, ST3, ST4, SOD, Vv, There were no significant differences in Sv, δ, number density (Nv), longitudinal diameter (Vd), ADP, AMP, and TNA. Visible, Ligustrazine can improve the mitochondrial function and structure of myocardial ischemia-reperfusion injury by reducing oxygen free radical levels and reducing calcium overload.