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目的观察抗坏血酸对大鼠再灌注心律失常在体模型影响,并探讨其作用机制。方法将52只雄性SD大鼠(220~250g)随机分为5组:假手术组(n=8),模型组(n=20),抗坏血酸25mg/kg组(n=8),50mg/kg组(n=8),100mg/kg组(n=8);通过结扎左冠状动脉前降支的方法建立再灌注心律失常模型,药物处理组于术前10min静脉注射抗坏血酸;分析再灌注期心电图变化及检测缺血心肌丙二醛含量。结果与模型组相比,50、100 mg/kg抗坏血酸显著改善了心律失常评分,缩短室颤时间,减少缺血心肌丙二醛含量。结论高剂量的抗坏血酸能明显减轻再灌注心律失常导致的损害,其作用机制可能与其提高心肌抗氧化能力,保持膜的稳定性和完整性相关。
Objective To observe the effect of ascorbic acid on reperfusion arrhythmia in rats and its mechanism. Methods Fifty-two male Sprague-Dawley rats (220-250g) were randomly divided into 5 groups: sham operation group (n = 8), model group (n = 20), ascorbic acid 25mg / kg group (N = 8) and 100 mg / kg group (n = 8). The model of reperfusion arrhythmia was established by ligation of left anterior descending coronary artery. The drug treatment group received intravenous injection of ascorbic acid 10 min before operation. The reperfusion electrocardiogram Changes and detection of myocardial MDA content. Results Compared with model group, 50,100 mg / kg ascorbic acid significantly improved arrhythmia score, shortened ventricular fibrillation time and decreased myocardial MDA. Conclusion High doses of ascorbic acid can significantly reduce the damage caused by reperfusion arrhythmia, and its mechanism may be related to its ability to increase myocardial antioxidant capacity and maintain membrane stability and integrity.