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背景:神经元的功能状态与机体的生命活动密切相关。目前已有多种实验方法来鉴定神经元轴突的结构和功能变化。但对于树突,人们给予的重视还很少。目的:观察电针损伤大鼠中脑后神经元微管相关蛋白2(MAP2)免疫反应变化。设计:设立对照的动物实验研究。地点和材料:地点为吉林大学中日联谊医院中心实验室。Wistar大鼠,雌雄不限,体质量(180±120)g,每组6只动物。干预:电针损伤大鼠中脑,不同时间取脑组织,行MAP2免疫组织化学染色。主要观察指标:神经元MAP2免疫反应及不同时间病理变化。结果:正常大鼠脑组织MAP2免疫反应发生在神经元的树突和胞体。电针损伤后可见MAP2免疫反应不同的神经元:固缩神经元,轻度受损神经元,正常神经元。结论:在中枢神经系统损伤实验性研究中,MAP2免疫反应是观察神经元功能状态的一项好指标。
Background: The functional status of neurons is closely related to the life activities of the body. There are a variety of experimental methods to identify changes in the structure and function of neuronal axons. But for dendrites, people pay little attention. Objective: To observe the changes of microtubule-associated protein 2 (MAP2) immunoreactivity in middle-posterior neurons of electroacupuncture rats. Design: Establishment of a Controlled Animal Experiment Study. Venue and Materials: The location is Central Laboratory of Sino-Japanese Friendship Hospital, Jilin University. Wistar rats, male or female, body weight (180 ± 120) g, 6 animals per group. Intervention: Electroacupuncture injury in rat midbrain, brain tissue taken at different times, line MAP2 immunohistochemical staining. MAIN OUTCOME MEASURES: Neuronal MAP2 immune response and pathological changes at different times. Results: The MAP2 immunoreactivity in normal rat brain tissue occurred in the dendrites and soma of neurons. Electroacupuncture injury can be seen after different MAP2 immune response neurons: pyramidal neurons, mildly damaged neurons, normal neurons. Conclusion: In the experimental study of central nervous system injury, MAP2 immune response is a good indicator to observe the functional status of neurons.