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1981年、1982年,应用clark结瘤及无瘤大豆同位基因系进行盆钵和田间试验.通过乙炔还原法查明大豆在开花期一鼓粒期大约50天期间内,其固氮活性最强.大豆的共生固氮量,通过乙炔还原法,“A_N”值法和全氮差异法测得的结果基本接近,在60～80公斤/公顷之间.约占大豆需氮量的1/4～1/3.还对固氮方法进行了讨论.大豆是我国东北地区主要作物之一,曾占主农区耕地面积的1/4左右.在以大豆为中心的有机农作体制下有利于保持土壤肥力,控制病虫灾害,调节农时劳力,保证人民营养和减轻环境污染.评价大豆在有机农业中的作用和地位时,人们首先注意到根瘤菌的共生固氮特性并着眼于固氮量的估计.Burtom等报导种一次大豆能固氮100公斤/公顷.Sears指出大豆根瘤菌的共生固氮是受土壤条件的影响自41～112磅/英亩/年,Weher等用结瘤与不结瘤同位基因系大豆进行试验指出.固氮量受施肥量影响,变动在1～142磅/英亩/年之间.为查明我省气候土壤条件下大豆生育期间根瘤固氮活性的动态变化,估测固氮数量,1981～1982年进行了盆钵和田间试验.
In 1981 and 1982, pot and field experiments were carried out using clark nodulation and tumor-free soybean alleles, and the acetylene reduction method was used to find that soybean had the highest nitrogen-fixing activity in about 50 days during the blooming stage. The symbiotic nitrogen fixation of soybean was nearly the same between “A_N” value method and total nitrogen difference method by acetylene reduction method, which was between 60 and 80 kg / ha, accounting for about 1/4 to 1 Nitrogen fixation methods are also discussed.Soybean is one of the main crops in northeastern China and has accounted for about 1/4 of the cultivated area in the main agricultural areas and is conducive to maintaining soil fertility under the organic farming system with soybean as the center , Controlling pests and diseases, adjusting labor force during farming, ensuring people’s nutrition and reducing environmental pollution.When evaluating the role and status of soybean in organic agriculture, people first noticed the symbiotic nitrogen fixation characteristics of Rhizobia and focused on the estimation of nitrogen fixation.Burtom et al Reported that once a soybean nitrogen fixation 100 kg / ha.Sears pointed out that the symbiotic nitrogen fixation of soybean rhizobia is affected by soil conditions from 41 to 112 pounds / acre / year, Weher and other nodulation and non-nodulation with soy strains were tested Pointed out that the amount of nitrogen fixation In order to find out the dynamic change of nitrogen fixation activity during the growth of soybean in the climate and soil conditions in our province, the amount of nitrogen fixation was estimated. From 1981 to 1982, Field experiment.