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为了探讨青石棉对肺损伤的机理 ,用兔肺泡巨噬细胞(AM)和人胚肺成纤维细胞 (HEPF)组成体外模型 ,采用MTT颜色反映法测定青石棉处理兔AM 2 4h后的培养上清液刺激HEPF细胞增殖情况 ,同时用标准石英作为阳性对照 ,二氧化钛作为阴性对照 ,以不用粉尘处理的AM培养上清液作为正常对照。结果发现青石棉能明显刺激HEPF增殖 ,呈剂量 效应关系 (P <0 0 1)。青石棉浓度在 10 0 μg/ml时 ,青石棉组HEPF的净增殖率达 6 0 91% ,是正常对照组的 3 48倍 ,高于阳性对照和阴性对照 10 0 μg/ml青石棉处理兔AM的培养上清液的不同稀释度对HEPF增殖的影响不同 ,随稀释度的加大 ,其促细胞增殖的能力越弱 ,以 1∶2稀释度作用最强 ,明显高于阳性对照和阴性对照 (P <0 0 1)。提示青石棉处理肺泡巨噬细胞的培养上清液中存在有促进肺成纤维细胞增殖的生物活性物质 ,若能阻止这些生物活性物质的合成和分泌将对石棉相关疾病的预防和治疗具有重要意义
In order to investigate the mechanism of crocidolite on lung injury, an in vitro model of rabbit alveolar macrophages (AM) and human embryonic lung fibroblasts (HEPF) was established. MTT colorimetric assay Serum HEPF cells proliferation stimulated by the same time with the standard quartz as a positive control, titanium dioxide as a negative control, without dust treatment AM supernatant as a normal control. The results showed that crocidolite could significantly stimulate the proliferation of HEPF in a dose-dependent manner (P <0.01). When the concentration of crocidolite was 100 μg / ml, the net growth rate of HEPF in crocidolite group was 6 0 91%, which was 3480 times higher than that of the normal control group and higher than that of the cocoa plant treated with 100 μg / ml crocidolite The effects of different dilutions of AM supernatant on the proliferation of HEPF were different. With the increase of dilution, the cell proliferation ability of AM was weakest, the effect of 1: 2 dilution was the strongest, which was significantly higher than the positive control and negative Control (P <0 0 1). Prompted crocidolite treatment of alveolar macrophages culture supernatant exists to promote the proliferation of pulmonary fibroblasts bioactive substances, if the prevention of the synthesis and secretion of these bioactive substances will be asbestos-related diseases prevention and treatment of great significance