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目的对黄牡固本丸建立有效的质量控制方法。方法采用薄层色谱法对白术、牡丹皮、大黄、淫羊藿进行定性色谱鉴别;采用薄层色谱扫描法测定了方中黄芪所含黄芪甲苷的含量。结果该方法专属性强,含量测定黄芪甲苷在0.54～4.32μg间呈现良好的线性关系,黄芪甲苷平均回收率为97.11%,RSD=1.35%;薄层色谱鉴别斑点清晰,无干扰,易分辨。结论该法准确、简便、重复性好,可用于黄牡固本丸的质量控制。
Objective To establish an effective quality control method for Huangmu Guben Wan. Methods TLC, Amaryllis, Moutan, Rhubarb, Epimedium were identified by qualitative chromatographic method. The content of Astragaloside contained in Radix Astragali was determined by TLC. Results The method was specific and specific. The content of astragaloside showed a good linear relationship in the range of 0.54 ~ 4.32μg. The average recovery of astragaloside was 97.11%, RSD = 1.35%. The TLC identified spots were clear and without interference. Distinguish. Conclusion The method is accurate, simple and reproducible. It can be used for the quality control of Huangmu Guben pill.